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Evaluating the performance of the Alere PBP2a SA Culture Colony Test with the Vitek 2 Antimicrobial Susceptibility Test Card System as reference standard in coagulase-negative species.

Lo TS, Goto M, Hammer KDP. Evaluating the performance of the Alere PBP2a SA Culture Colony Test with the Vitek 2 Antimicrobial Susceptibility Test Card System as reference standard in coagulase-negative species. Infectious medicine. 2024 Sep 1; 3(3):100126.

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Abstract:

BACKGROUND: The Alere PBP2a SA Culture Colony Test is an FDA-cleared immunochromatographic assay for rapid detection of penicillin-binding protein2a (PBP2a) in . METHODS: We investigated the performance of the PBP2a SA Culture Colony Test with 78 coagulase-negative (CoNS) isolates from different body sites, with the Vitek 2 Antimicrobial Susceptibility Test (AST) as a reference standard. RESULTS: The CoNS species were 62 ; 6 ; 3 ; 2 ; 2 ; and 1 each of and . Of the 78 CoNS isolates, 68 showed concordance in the PBP2a IC assay and Vitek 2 AST. Discordance was seen for 10 isolates, which showed negative in the PBP2a assay, despite oxacillin-resistance detection using the Vitek 2 AST (66.7% sensitivity and 100% specificity). All non- CoNS were identified with 100% concordance using the PBP2a IC assay and Vitek 2 AST. CONCLUSION: We demonstrated that, while the PBP2a IC assay has low sensitivity in determining the susceptibility of to oxacillin, it highly accurately predicted the susceptibility of non- CoNS to oxacillin. The diagnostic accuracy for non- CoNS needs further assessment with more isolates to confirm our findings.




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