Talk to the Veterans Crisis Line now
U.S. flag
An official website of the United States government
Veterans Benefits and Health Care About VA Find a VA Location

VA Health Systems Research

Go to the VA ORD website
Go to the QUERI website

HSR&D Citation Abstract

Search | Search by Center | Search by Source | Keywords in Title

Development of Phage Cocktails to Treat Catheter-Associated Urinary Tract Infection and Associated Biofilms.

Sanchez BC, Heckmann ER, Green SI, Clark JR, Kaplan HB, Ramig RF, Hines-Munson C, Skelton F, Trautner BW, Maresso AW. Development of Phage Cocktails to Treat Catheter-Associated Urinary Tract Infection and Associated Biofilms. Frontiers in microbiology. 2022 May 10; 13:796132.

Dimensions for VA is a web-based tool available to VA staff that enables detailed searches of published research and research projects.

If you have VA-Intranet access, click here for more information vaww.hsrd.research.va.gov/dimensions/

VA staff not currently on the VA network can access Dimensions by registering for an account using their VA email address.    Search Dimensions for VA for this citation
* Don't have VA-internal network access or a VA email address? Try searching the free-to-the-public version of Dimensions


Abstract:

High rates of antimicrobial resistance and formation of biofilms makes treatment of catheter-associated urinary tract infections (CAUTI) particularly challenging. CAUTI affect 1 million patients per year in the United States and are associated with morbidity and mortality, particularly as an etiology for sepsis. Phage have been proposed as a potential therapeutic option. Here, we report the development of phage cocktails that lyse contemporary strains isolated from the urine of patients with spinal cord injury (SCI) and display strong biofilm-forming properties. We characterized phage against biofilms in two CAUTI models. Biofilm viability was measured by an MTT assay that determines cell metabolic activity and by quantification of colony forming units. Nine phage decreased cell viability by > 80% when added individually to biofilms of two strains in human urine. A phage cocktail comprising six phage lyses 82% of the strains in our library and is highly effective against young and old biofilms and against biofilms on silicon catheter materials. Using antibiotics together with our phage cocktail prevented or decreased emergence of resistant to phage in human urine. We created an anti-biofilm phage cocktail with broad host range against strains isolated from urine. These phage cocktails may have therapeutic potential against CAUTI.




Questions about the HSR website? Email the Web Team

Any health information on this website is strictly for informational purposes and is not intended as medical advice. It should not be used to diagnose or treat any condition.